Translational Landscape of Extracellular Vesicle Research
Biological Properties and Mechanisms of Action of Extracellular Vesicles
Yáñez-Mó M, et al. 2015: Biological properties of extracellular vesicles and their physiological functions. J Extracell Vesicles. PMID: 25979354
Perissinotto F, et al. 2021: Structural insights into fusion mechanisms of small extracellular vesicles with model plasma membranes. Nanoscale. PMID: 33687046
Grava M, et al. 2022: Calorimetry of extracellular vesicles fusion to single phospholipid membrane. Biomol Concepts. PMID: 35312244
Priglinger E, et al. 2020: SVF-derived extracellular vesicles carry characteristic miRNAs in lipedema. Sci Rep. PMID: 32350368
Manufacturing, Standardization, and Quality Assessment of Therapeutic Extracellular Vesicles
Pachler K, et al. 2017: A Good Manufacturing Practice-grade standard protocol for exclusively human mesenchymal stromal cell-derived extracellular vesicles. Cytotherapy. PMID: 28188071
Pachler K, et al. 2017: An In Vitro Potency Assay for Monitoring the Immunomodulatory Potential of Stromal Cell-Derived Extracellular Vesicles. PMID: 28671586
Witwer KW, et al. 2019: Defining mesenchymal stromal cell (MSC)-derived small extracellular vesicles for therapeutic applications. J Extracell Vesicles. PMID: 31069028
Gimona M, et al. 2021: Critical considerations for the development of potency tests for therapeutic applications of mesenchymal stromal cell-derived small extracellular vesicles. Cytotherapy. PMID: 33934807
Priglinger E, et al. 2021: Label-free characterization of an extracellular vesicle-based therapeutic. J Extracell Vesicles. PMID: 34669269
Lau HC, et al. 2022: GMP-compliant manufacturing of biologically active cell-derived vesicles produced by extrusion technology. J Extracell Biol. PMID: 38938599
Preclinical Regenerative and Immunomodulatory Applications of Extracellular Vesicles
Romanelli P, et al. 2019: Extracellular Vesicles Can Deliver Anti-inflammatory and Anti-scarring Activities of Mesenchymal Stromal Cells After Spinal Cord Injury. Front Neurol. PMID: 31849808
Romanelli P, et al. 2022: Enhancing Functional Recovery Through Intralesional Application of Extracellular Vesicles in a Rat Model of Traumatic Spinal Cord Injury. Front Cell Neurosci. PMID: 35046776
Warnecke A, et al. 2020: Extracellular vesicles from human multipotent stromal cells protect against hearing loss after noise trauma in vivo. Clin Transl Med. PMID: 33377658
Jenner F, et al. 2023: Evaluation of the Potential of Umbilical Cord Mesenchymal Stromal Cell-Derived Small Extracellular Vesicles to Improve Rotator Cuff Healing: A Pilot Ovine Study. Am J Sports Med. PMID: 36645050
Deluca A, et al. 2024: Synergistic effect of umbilical cord extracellularvesicles and rhBMP-2 to enhance the regeneration of a metaphyseal femoral defect in osteoporotic rats. Stem Cell Res Ther. PMID: 38764077
Deininger C, et al. 2025: Anatomical implant region – a critical determinant for the osteogenic potency of small extracellular vesicles and rhBMP-2. Eur J Trauma Emerg Surg. PMID: 41410757
Clinical Translation: First-in-Human Applications of Extracellular Vesicles
Warnecke A, et al. 2021: First-in-human intracochlear application of human stromal cell-derived extracellular vesicles. J Extracell Vesicles. PMID: 34136108
Krause M, et al. 2025: First-In-Human Application of Human Umbilical Cord-Derived Extracellular Vesicles in Tethered Spinal Cord Release Surgery. J Extracell Vesicles. PMID: 40536443
Therapeutic Use of Extracellular Vesicles – Concepts and Perspectives
Gimona M, et al. 2017: Manufacturing of Human Extracellular Vesicle-Based Therapeutics for Clinical Use. Int J Mol Sci. PMID: 28587212
Reiner AT, et al. 2017: Concise Review: Developing Best-Practice Models for the Therapeutic Use of Extracellular Vesicles. Stem Cells Transl Med. PMID: 28714557
Rohde E, et al. 2019: Manufacturing and characterization of extracellular vesicles from umbilical cord-derived mesenchymal stromal cells for clinical testing. Cytotherapy. PMID: 30979664
Marolt Presen D, et al. 2019: Mesenchymal Stromal Cell-Based Bone Regeneration Therapies: From Cell Transplantation and Tissue Engineering to Therapeutic Secretomes and Extracellular Vesicles. Front Bioeng Biotechnol. PMID: 31828066
Warnecke A, et al. 2022: Extracellular Vesicles in Inner Ear Therapies-Pathophysiological, Manufacturing, and Clinical Considerations. J Clin Med. PMID: 36556073
Gil Izquierdo S, et al. 2025: Advances in extracellular vesicle-based nanomedicine for regenerative orthopaedics. J Nanobiotechnology. PMID: 41392147
Standards, Guidelines, and Regulatory Developments in the EV Field
Lener T, et al. 2015: Applying extracellular vesicles based therapeutics in clinical trials – an ISEV position paper. J Extracell Vesicles. PMID: 26725829
Théry C, et al. 2018: Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. J Extracell Vesicles. PMID: 30637094
Börger V, et al. 2020: International Society for Extracellular Vesicles and International Society for Cell and Gene Therapy statement on extracellular vesicles from mesenchymal stromal cells and other cells: considerations for potential therapeutic agents to suppress coronavirus disease-19. Cytotherapy. PMID: 32425691
Paolini L, et al. 2022: Large-scale production of extracellular vesicles: Report on the „massivEVs“ ISEV workshop. J Extracell Biol. PMID: 38939213
Toh WS, et al. 2023: A report on the International Society for Cell & Gene Therapy 2022 Scientific Signature Series, „Therapeutic advances with native and engineered human extracellular vesicles“. Cytotherapy. PMID: 36931996
Shekari F, et al. 2023: Cell culture-derived extracellular vesicles: Considerations for reporting cell culturing parameters. J Extracell Biol. PMID: 38939735
Gurriaran-Rodriguez U, et al. 2026: From Lab to Industry: Advancing the Translational Potential of Extracellular Vesicles Through the ISEV Translation, Regulation and Advocacy Committee (ISEV-TRA). J Extracell Vesicles. PMID: 41531372